Thank you very much for the analysis. I'll definitely set the minimum star HFD to avoid guiding on hot pixels. I was on bin2 since when I first set up this image train several months ago I was having problems finding guide stars and bin2 seemed to work. I'll go back to bin1 and see how it goes. I normally guide with an exposure of 3 seconds; not sure why I had 1 second in that series.
Greatly appreciate the help.